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Probetex,Inc 肾炎模型诱导试剂一级代理
时间:2019-12-30作者:biolead来源:biolead关注:

特色亚博bob体育:

绵羊抗大鼠肾小球基底膜血清(PTX-001AGBM)
Sheep Anti Rat Glomerular Basement Membrane (GBM) Serum
--抗GBM型肾炎模型诱导

 

亚博bob体育名称

Sheep Anti Rat Glomerular Basement Membrane (GBM) Serum

货号

PTX-001AGBM

规格

25ml

保存

-20℃

说明

亚博bob体育经56℃热处理30min,请避免反复冻融。解冻后会有轻微沉淀,注射前3000-5000rmp 离心30min。

注:只做过大鼠炎症,没有做过小鼠验证。


应用说明:(实验前请仔细阅读实验流程)

每瓶血清含有足够的抗体用于诱导至少25只大鼠*(175-200 g体重)被动抗GBM肾炎模型。
免疫抗GMB血清:麻醉大鼠,尾静脉注射抗GMB血清超过15秒,疾病的产生依赖于免疫剂量,依照免疫剂量完全打入体内很关键(推荐用量:0.4-0.5ml/100g体重)。由于不同批次抗血清差异,大鼠来源,以及实验要求的不同,亚博bob体育建议进行剂量-反应研究,具体的免疫剂量和疾病的严重程度,需要根据每个不同批次血清和产生疾病的严重程度,选择合适的免疫剂量。

肾病研究:

1)异源性疾病:免疫抗GBM (anti GBM)3-5天后,免疫荧光检测显示绵羊IgG在肾小球基底膜内呈线状沉积。大鼠c3也呈线性分布,24小时后出现蛋白尿。
2)自身疾病:免疫抗GBM抗体8-10天后,检测大鼠蛋白尿增加,同时大鼠肾小球IgG(自身抗体) 呈线状沉积,自体疾病变得明显。(自体)免疫组化呈线性模式的IgG(图1)。到3周时,蛋白尿有望达到200毫克/24小时,肾小球病变(图2)明显,随着时间的推移,严重程度增加,导致肾小球硬化。

图1.免疫荧光检测显示免疫抗GBM3周后肾小球毛细血管壁大鼠IgG免疫沉积的。

图2.免疫组化染色显示免疫抗GBM 3周后新月体形成、系膜细胞增生、毛细血管变形。

相关亚博bob体育:

货号

亚博bob体育名称

规格

肾炎模型

PTX-001AGBM

Sheep Anti-Rat GBM Serum 

25ml

PTX-002S

Sheep Anti-Rat Fx1A Serum

25ml

PTX-003S

Sheep Anti-Rat Thy-1 Serum

20ml

PTX-000S

Sheep Non-Immune Serum

25ml

组织亚博bob体育(动物疾病模型组织、切片、血浆、尿液)

PTX-001

Anti-GBM (Immune-mediated Glomerulonephritis)

-

PTX-002

Anti-Fx1A (Heymann Nephritis, Membranous Nephritis)

-

PTX-003.

Anti-Thy-1 (Mesangial Proliferative Glomerulonephritis) 

-

PTX-004

Diabetic nephropathy (streptozotocin- Type I Diabetes) 

-

PTX-005

Control (Normal) Kidney

-

PTX-006

Sheep Kidney Section(anti-GBM and Fx1A membranous nephropathy

-

细胞

CRGMes-1

Rat Glomerular Mesangial Cells

1x106

CRGEp-1

Rat Glomerular Epithelial Cells

1x106

CBGEn-1

Bovine Glomerular Endothelial Cells

1x106

CMUB-1

Mouse Ureteric Bud Cells

1x106

CMMM-1

Mouse Metanephric Mesynchymal Cells

1x106

CRMM-1

Rat Metanephric Mesynchymal Cells

1x106  

抗体

BS-001GBM

FITC- Sheep Anti-Glomerular Basement Membrane IgG

0.5ml

BS-002ECM

FITC- Sheep Anti-Extracellular Matrix (ECM) IgG

0.5ml
 


参考文献:

1.McIntosh LM, Barnes JL, Barnes VL, McDonald JR. Selective CCR2-targeted macrophage depletion ameliorates experimental mesangioproliferative glomerulonephritis. Clin Exp Immunol 155:295-303, 2009. Osprey Pharma, Probetex, Inc. (Contract Research, Pre-Clinical Testing, Histopathology)

2.Velagapudi C, Nilsson R-P, Barnes VL, Arar M, Abboud HE, Barnes JL. Reciprocal induction of simple organogenesis by mouse kidney progenitor cells in three-dimensional co-culture. Am J Pathol 180:819-830, 2012. Link (Cover Photo, February Issue). Probetex, Inc, Univ TX Hlth Sci Ctr, San Antonio, TX.  (CMMM-1 and CMUB-1: Mouse Kidney Primordial Cells).

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5.Patel M, Velagapudi C, Burns HS, Doss R, Lee, MJ, Mariappan M, Wagner B, Arar M, Barnes VL, Abboud HE, Barnes JL. Mouse metanephric mesenchymal cell-derived angioblasts undergo vasculogenesis in three-dimensional culture. Am J Pathol 188: 768-784, 2018. Probetex, Inc, Univ TX Hlth Sci Ctr, San Antonio, TX. (CMMM-1 and CMUB-1: Mouse Kidney Primordial Cells).

6.Rufanova VA, Lianos E, Alexanian A, Sorokina E, Sharma M, McGinty A, Sorokin A: G3G overexpression in glomerular epithelial cells during anti-GBM-induced glomerulonephritis. Kidney Int 75:31-40, 2009. (mice) (Medical College of Wisconsin).

7.Lichtnekert J, Kulkarni OP, Mulay SR, Rupanagudi KV, Ryu M, Allam R, Vielhauer V, Muruve D, Lindenmeyer MT, Cohen CD, Anders H-J. Anti-GBM glomerulonephritis involves IL-1 but is independent of NLRP3/ASC inflammasome-mediated activation of caspase-1. PLoS ONE 6(10): e26778. doi:10.1371/journal.pone.0026778. October 2011. (mice) (Ludwig-Maximillians-Univeristy of Munich).

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10.Zheng W, Warner R, Ruggeri R, Su C, Cortes C, Skoura A, Ward J, Ahn K, Kalgutkar A, Sun D, Maurer TS, Bonin PD, Okerberg C, Bobrowski W, Kawabe T, Zhang Y, Coskran T, Bell S, Kapoor B, Johnson K, Buckbinder L. PF-1355, a mechanism-based myeloperoxidase inhibitor, prevents immune complex vasculitis and anti–glomerular basement membrane glomerulonephritis. J Pharmacol Exp Ther 353:288–298, 2015. (mice). Pfizer and University of Michigan.

11.Pavkovic M, Riefke B, Frisk AL, Gröticke I, Ellinger-Ziegelbauer H. Glomerulonephritis-induced changes in urinary and kidney MicroRNA profiles in rats. Toxicol Sci. 145(2), 348–359, 2015. (Rats). Bayer Pharma AG and Harvard Medical School.

12.Kumar SV, Kulkarni OP, Mulay SR, Darisipudi MN, Romoli S, Thomasova D, Scherbaum CR, Hohenstein B, Hugo C, Müller S, Liapis H, Anders HJ. Neutrophil Extracellular Trap-Related Extracellular Histones Cause Vascular Necrosis in Severe GN. J Am Soc Nephrol. 26 (10):2399-2413, 2015 (mice) (Ludwig-Maximilians-University of Munich, Munich).

13.Bosma M, Gerling M, Pasto J, Georgiadi A, Graham E, Shilkova O, Iwata Y, Almer S, Soderman J, Toftgard R, Wermeling F, Bostrom EA, Bostrom PA. FNDC4 acts as an anti-inflammatory factor on macrophages and improves colitis in mice. Nature Commun 7: 11314. Apr 12 2016. (mice) Karolinska Institute, Stockholm Sweden

14.Mulay SR, Romoli S, Desai J, Honarpischeh, MM, Kumar SV, Anders H-J. Murine double minute-2 inhibition ameliorates established crescentic glomerulonephritis. Am J Pathol. 186: 1442-153, 2016. (mice) Medizinische Klinik und Poliklinik University Hoispital of Ludwig-Maximillians-University , Munich, Germany.

15.Hachmo Y, Kalechman Y, Skornick I, Gafter U, Caspi RR, and Sredni B: The small tellurium compound AS101 ameliorates rat crescentic glomerulonephritis: association with inhibition of macrophage caspase-1 activity via very late antigen-4 inactivation. Front Immunol. 8: 240-, 2017. doi: 10.3389/immu.2017.00240 PMCID: PMC5339302. (Rats) C.A.I.R. Institute, The Safdiè AIDS and Immunology Research Center; Laboratory of Immunology, National Eye Institute, National
Institutes of Health, Bethesda, MD, USA

16.Hsu M-F, Betaieb A, Ito Y, Graham J, Havel PJ, Haj FG. Protein tyrosine phosphatase Shp2 deficiency in podocytes attenuates lipopolysaccharide-induced proteinuria. Scientific Reports 7: 461 2017. (mice). UC-Davis Univ Tennessee-Knoxville Tennessee, USA.

17.Velez JCQ, Arif E, Rodgers J, Hicks MP, Arthur JM, Nihalani D, Bruner ET, Budisavljevic MN, Atkinson C, Fitzgibbon WR, Janech MG. Deficiency of the angiotensinase aminopeptidase A increases susceptibility to glomerular injury. J Am Soc Nephrol: 28(7):2119-2132, 2017. (mice). Ochsner Clinic Foundation, New Orleans, LA, Medical University of South Carolina, Charleston, SC, Augusta University, Augusta, GA, University of Arkansas for Medical Sciences, Little
Rock, AR

18.Bettaieb A, Koike S, Chahed S, Zhao Y, Bachaalany S, Hashoush N, Graham J, Fatima H, Havel PJ, Gruzdev A, Zeldin DC, Hammock BD, Haj FG. Podocyte-specific soluble epoxide hydrolase deficiency in mice attenuates acute kidney injury. FEBS J. 284(13):1970-1986, 2017.(mice). UC Davis, Univ of Tenn-Knoxville, NIEHS PMCID:

19.Wen Y, Lu X, Ren J, Privratsky JR, Yang B, Rudemiller NP, Zhang J, Griffiths R, Jain MK, Nedospasov SA, Liu BC, Crowley SD. KLF4 in macrophages attenuates TNFα-mediated kidney injury and fibrosis. J Am Soc Nephrol.  30:1925-1938, 2019. (mice). Duke University Medical Center, Duke-National University of Singapore, Durham Veterans Affairs Medical Center.

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